Pengaruh Lamanya Penyimpanan Serum pada Suhu 20-80 0c Selama Satu Minggu terhadap Kadar Kolesterol Total

Abstract

Abstract: Laboratory examinations are essential to establish diseases diagnosis so the results should be accurate. Therefore, it is necessary to control the pre-analytic, analytic and post-analytic. Cholesterol examination is one of the ways to establish the diagnosis of DM, Heart and hypertension. This study was aimed to determine the effect of duration of serum storage at a temperature of 2°-8°C for a week with total cholesterol levels. Samples were checked daily for a week. The samples of this study were the 2^nd and 3rd grade of Health Analyst’s students who lived in the male dormitory where total samples were 5 samples. The study was conducted from March to June 2013. Each sample was checked for total serum cholesterol levels which stored at a temperature of 2°-8°C for a week. This examination used CHOD-PAP method. From the results of data analysis with Friedman Test method, it was determined that total cholesterol levels in serum which stored at temperature 2°-8°C for a week obtained Chi-Square value = 28.827 and p = 0.000 where showed that there was effect of duration of serum storage at temperature 2°-8°C for a week with total cholesterol level.

Abstrak: Pemeriksaan laboratorium sangat penting untuk menegakkan diagnosa penyakit sehingga hasil harus akurat. Oleh karena itu, perlu dilakukan pengendalian terhadap pra-analitik, analitik dan pasca analitik. Pemeriksaan kolesterol merupakan salah satu upaya untuk menegakkan diagnosa penyakit DM, Jantung, dan hipertensi. Penelitian ini bertujuan untuk mengetahui pengaruh lamanya penyimpanan serum pada suhu 2°- 8°C selama 1 minggu terhadap kadar kolesterol total. Sampel diperiksa setiap hari selama 1 minggu. Sampel penelitian ini adalah mahasiswa tingkat 2 dan 3 Analis Kesehatan yang tinggal di asrama putra dengan jumlah sampel sebanyak 5 sampel. Penelitian ini dilaksanakan pada bulan Maret-Juni 2013. Masing-masing sampel diperiksa kadar kolesterol total pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu. Pemeriksaan ini menggunakan metode CHOD-PAP. Dari hasil analisis data dengan metode Friedman Test, diketahui bahwa kadar kolesterol total pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu didapatkan besaran nilai Chi Square = 28,827 dan p = 0.000. Hasil uji signifkansi Chi Square metode Friedman test menunjukkan bahwa p < 0.05 sehingga dapat disimpulkan bahwa terdapat pengaruh lamanya penyimpanan pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu terhadap kadar kolesterol total.