Anti-Inflammatory Activity Test of Tapak Liman (Elephantopus scaber L.) Leaf Extract Using  Red Blood Cell Membrane Stabilization Method

Wahdaniah Wahdaniah; Rahmi Yanti; Laila Kamilla; Maulidiyah Salim

doi:10.30602/jlk.v9i1.2135

Anti-Inflammatory Activity Test of Tapak Liman (Elephantopus scaber L.) Leaf Extract Using Red Blood Cell Membrane Stabilization Method

Wahdaniah Wahdaniah, Rahmi Yanti, Laila Kamilla, Maulidiyah Salim

Abstract

Inflammation is the reaction of living tissue to trauma or infection, both in acute and chronic conditions. One plant with anti-inflammatory potential is the tapak liman leaf (Elephantopus scamber L.), which contains secondary metabolites such as phenolics, flavonoids, tannins, alkaloids, steroids/treponoids, and saponins. The purpose of this study was to determine the anti-inflammatory activity of Elephantopus scamber L. leaf extract at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08% using the red blood cell membrane stability method. Anti-inflammatory activity showed that 70% ethanol extract of tapak liman leaves had low anti-inflammatory activity at a concentration of 0.005% with a stability of 98.00%, while the highest activity was found at a concentration of 0.08% with a stability of 99.65%. Microscopic examination showed that at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08%, the administration of tapak liman extract could restore the normal shape of erythrocytes. The Kruskal-Wallis test yielded a significance value of 0.000, which is less than 0.05, meaning that Ha is accepted. It can be concluded that there are differences in the anti-inflammatory activity of tapak liman leaf extract (Elephantopus scamber L.) at concentrations of 0.005%, 0.01%, 0.02%, 0.04%, and 0.08%.

Keywords

Anti-inflammatory, Tapak Liman Leaves, Red Blood Cells

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DOI: https://doi.org/10.30602/jlk.v9i1.2135