Daya Hambat Konsentrasi Air Rebusan Cacing Tanah (Lumbricus Rubellus) Terhadap Pertumbuhan Bakteri Salmonella Typhi Dengan Metode Difusi

Abstract

Abstract: Earthworms (Lumricus rubellus) is included in avertebrates animal (invertebrates) so they usually called soft animal. Earthworms (Lumricus rubellus) have lumbrokinases, peroksidase, catalase, cellulose and arhidonic acid which have capacity for reducing fever due to bacteria infection. Study objective was to identify potential concentration of earthworms stew water against Salmonella typhi advancement by using diffusion method. This study used laboratory experimental design with purposive sampling method, a determining sampling by selecting a sample within population based on desired research. This study resulted from 3 treatments with 30 measured total samples obtained minimum concentration was 20% which had 8 mm diameter of inhibitory zone, meanwhile 100% was the maximum concentration with 21 mm as diameter of inhibitory zone. According to statistical test, One Way ANova, gained value (p = 0,00 < 0,05) so H0 refused and Ha accepted, meaning that there was different inhibitory potency in each earthworms stew water in order to constrain Salmonella thypi growth with diffusion method applied. Conclusion from this study was the higher concentration of earthworms stew water, the higher inhibitory zone formed around paper disk.

Abstrak: Cacing tanah (Lumricus rubellus) adalah tergolong dalam kelompok binatang avertebrata (tidak bertulang belakang) sehingga sering disebut binatang lunak. Cacing tanah (Lumricus rubellus) mengandung enzim lumbrokinase, peroksidase, katalase, dan selulosa. serta asam arhidonat yang mempunyai kemampuan menurunkan panas badan (deman) yang disebabkan infeksibakteri. Tujuan penelitian adalah untuk mengetahui daya konsentrasi air rebusan cacing tanah (Lumbricus rubellus) terhadap pertumbuhan bakteri Salmonella typhi. Dengan metode difusi. Desain penelitian yang digunakan dalam penelitian ini adalah penelitian eksperimental laboratorium, dengan metode pengambilan sampel purposive sampling, yaitu suatu penetapan sampel dengan cara memilih sampel diantara populasi sesuai dengan yang dikehendaki penelitian. Hasil penelitian dari 3 perlakuan dengan 30 sampel dapat diketahui konsentrasi minimum 20% dengan diameter zona hambat 8 mm sedangkan konsentrasi maksimun 100% dengan diameter zona hambat 21 mm. Berdasarkan uji statistik menggunakanAnova One Wey diperoleh nilai (p = 0,00 < 0,05) maka HO ditolak dan Ha diterima, berarti terdapat perbedaan daya hambat air rebusan cacing tanah (Lumbricus rubellus) terhadap pertumbuhan bakteri Salmonella typhi dengan metode difusi. Kesimpulan dari hasil penelitian yaitu semakin tinggi konsentrasi air ribusan cacing tanah, maka semakin besar zona hambat yang terbentuk disekitar paper disk cakram.