Daya Hambat Perasan Bawang Dayak (Eleutherine Palmifolia (L.) Merr) Terhadap Pertumbuhan Bakteri Stapylococcus aureus Metode Difusi

Laila Kamilla, Sri Tumpuk, Maulidiyah Salim

Abstract


Traditional medicine is an ingredient that can be derived from plants, animals, minerals, preparations of extract (galenic) or a mixture of these materials which have been used for generations for treatment. The use of medicinal plants as traditional medicine has many advantages. It is easy to obtain because the raw materials can be grown and mixed by yourself, so it is cheaper. People also consider traditional medicine to be safer than chemical drugs because the side effects are relatively smaller.

One of the plants that have been used as traditional medicine is the Dayak onion. Dayak onion (Eleutherine americana L. Merr) is empirically used by local people in Kalimantan as a traditional medicinal plant. Dayak onions can be used as raw materials for medicine because they contain active compounds in the form of alkaloids, glycosides, flavonoids, phenolics, triterpenoids or steroids and anthraquinones which have been known as medicinal raw materials. Until now, the Dayak onion plant is used for the treatment of colon cancer, breast cancer, diabetes mellitus, hypertension, lowering cholesterol, stroke, stomach pain medication after childbirth and ulcer medicine.

This study aims to determine the inhibition of Dayak onion (Eleutherine americana L. Merr) on the growth of Stapylococcus aureus bacteria by diffusion method. The research design was a quasi-experimental. The population of Dayak onion bulbs. As a sample of Dayak onion bulbs made in concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. With the formula of Randomized Block Design (RAK), 30 samples were obtained. Antimicrobial testing using the diffusion method.

The results obtained were resistant at a concentration of 10% - 60% (inhibition zone < 9 mm) and a concentration of 70-90% intermediate (inhibition zone 10-11) and a concentration of 100% sensitive (inhibition zone > 12). After being analyzed statistically using the Kruskal-Wallis test, a significant asymp value was obtained. sig = 0.001 < 0.050. It was concluded that there was an effect of the juice of the Dayak onion (Eleutherine palmifolia (L.) Merr) in inhibiting the growth of Stapylococcus aureus bacteria.


Keywords


Juice; Dayak Onion

References


Andika, F. (2010). Pengaruh Perasan Air Kulit Jeruk Manis (Citrus Aurantium Sub Spesies Sinensis) Terhadap Tingkat Kematian Larva Aedes aegeypti Instar III IN VITRO. Fakultas Kedokteran Universitas Sebelas Maret Surakarta.

Cahaya T. 2011, Kumpulan Obat Tradisional Nusantara, Cetakan III, Rama Edukasitama, Jakarta.

Galingging, R.Y. 2009. Bawang Dayak (Eleutherine palmifolia) Sebagai Tanaman Obat Multifungsi. Warta Penelitian dan Pengembangan15 (3):

-4.

Harborne, J.B. 2006, Metode Fitokimia: Penuntuncara Modern Menganalisis Tumbuhan, ITB. Bandung.

Indrawati, N. L., Farm, S., & Razimin, S. S. 2013, Bawang Dayak: Si Umbi Ajaib Penakluk Aneka Penyakit, AgroMedia, Jakarta.

Jawetz, Melnick, & Adelberg., 2014, Mikrobiologi Kedokteran, edisi 25, dr. Aryandhito widhi nugroho, et al., Buku Kedokteran EGC, Jakarta.

Kementrian Kesehatan Republik Indonesia. 2013,Rencana Induk Pengembangan Bahan Baku Obat Tradisional, Diakses dari:https://www.kemhan.go.id/itjen/wpcontent/uploads/migrasi/peraturan/88.pdf

Kementrian Kesehatan Republik Indonesia. 2012, Registrasi Obat Tradisional, Diakses dari: http://sireka.pom.go.id/requirement/PMK-7-2012-Registrasi-Obat-Tradisional.pdf.

Kuswiyanto., 2016. Bakteriologi 2 : Buku Ajar Analis Kesehatan. Buku Kedokteran EGC. Jakarta.

Latief, A. 2012,Obat Tradisional. EGC, Jakarta.

Mangan, Yelia. 2003. Cara Bijak Menaklukan Kanker. Agromedia. Jakarta

Pratiwi, S. T., 2008, Mikrobiologi Farmasi, Erlangga, Yogyakarta.

Saputra, A. 2015, Uji Aktivitas Antiinflamasi Ekstrak Etanol 96% Kulit Batang Kayu Jawa (Lannea coromandelica) dengan Metode Stabilisasi Sel Darah Merah secara In vitro, Skripsi, UIN Syarif Hidayatullah, Jakarta.

Sari, Y.P., 2012, Efektifitas Air Perasan Daun Pepaya (Carica papaya L) dalam Menghambat Pertumbuhan Bakteri Staphylococcus aureus, KTI, Politeknik Kesehatan Jurusan Analis Kesehatan, Pontianak.

Sibagariang, E. E., Julianie., Rismalinda dan Siti N. 2010,Metodologi Penelitian Untuk Mahasiswa Diploma Kesehatan, Cetakan I, TIM, Jakarta.

Styawati, E.S.S. 2013.Daya Hambat Perasan Daun Nilam (Pogostemon Sp.) Terhadap Pertumbuhan Bakteri Penyebab Bisul, Jurusan Farmasi Politeknik Kesehatan Makassar.

Sugiyono., 2011, Metode Penelitian Kuantitatif Dan Kualitatif Dan R&D, Alfabeta, Bandung.

Yuniart T. 2008. Ensiklopedia Tanaman Obat Tradisional. Media Presindo, Jakarta.




DOI: https://doi.org/10.30602/jlk.v5i2.977

Article Metrics

Abstract views : 69|

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Jurnal Laboratorium Khatulistiwa

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

JURNAL LABORATORIUM KHATULISTIWA INDEXED BY:

   

LICENSE JOURNAL:

Creative Commons License
Jurnal Laboratorium Khatulistiwa is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

VISITOR STAT:

Media Laboratorium Khatulistiwa