Perbedaan Perasan dan Rebusan Daun Cengkodok (Melastoma malabathricum L.) dalam Menghambat Pertumbuhan Jamur Trichophyton rubrum

Mardiana Mardiana, Etiek Nurhayati, Supriyanto Supriyanto

Abstract


Abstract: Cengkodok leaves (Melastoma malabtahricum L.) is one of plant that has usefulness like medicine and has the potential to be researched. One of the usefulness of treating skin diseases caused by Trichophyton rubrum fungus. The research aims to determine the defference of juice and boiled of cengkodok leaves (Melastoma malabathricum L.) in inhibiting the growth of Trichophyton rubrum fungus. Each treatment of juice and stew has concentrations of 10%, 20%, 30%, 40%, 50%, 60%,70%, 80%, 90%, 100%. The research method is a quasi experiment, sampling technique by purposive sampling, and use method of solid dilution using PDA (Potato Dextrose Agar) with 3 replications. The results of the research on the juiced treatment that concentration of 10% have an average growth 261 CFU. The higher concentration of antifungi ingredients then the number of fungal colonies growth decreases until concentration of 100% have an average growth 12 CFU. The 10% concentration boiled treatment there was an average growth of 205 CFU and the number of fungal colonies growth decreases at concentration of 100% have an average growth 1 CFU. Effective concentration of cengkodok leaves juiced (Melastoma malabathricum L.) starts from concentration of 60% with inhibiting Trichophyton rubrum growth fungus 52,77% and boiled start from concentration 50% with percentage of inhibiting 59,41%. The data obtained were analyzed statistically using independent t test obtained p value (Sig. 2 tailed) 0.116 > 0.05. The results showed no difference between the juice and boiled of Cengkodok leaves (Melastoma malabathricum L.) in inhibiting Trichophyton rubrum fungus.

Abstrak: Daun cengkodok (Melastoma malabathricum L.) merupakan tumbuhan berkhasiat obat, salah satu khasiatnya yakni mengobati penyakit kulit yang diakibatkan oleh Jamur Trichophyton rubrum. Penelitian ini bertujuan untuk mengetahui perbedaan perasan dan rebusan daun cengkodok (Melastoma malabathricum L.) dalam menghambat pertumbuhan jamur Trichophyton rubrum. Masing-masing perlakuan memiliki konsentrasi 10%, 20%, 30%, 40%, 50%, 60%,70%, 80%, 90%, 100%. Metode penelitian ini adalah Quasi Experiment, teknik pengambilan sampel dengan cara purposive sampling, dan menggunakan metode dilusi padat menggunakan media PDA (Potato Dextrose Agar) dengan 3 replikasi. Hasil penelitian pada perlakuan perasan konsentrasi 10% terdapat rata-rata pertumbuhan jumlah koloni jamur yakni 261 CFU. Semakin tinggi konsentrasi bahan antifungi maka jumlah pertumbuhan koloni jamur semakin berkurang hingga pada konsentrasi 100% terdapat rata-rata jumlah koloni jamur yakni 12 CFU. Pada perlakuan rebusan konsentrasi 10% terdapat rata-rata pertumbuhan jumlah koloni jamur yakni 205 CFU dan semakin berkurang jumlah pertumbuhan koloni jamur pada konsentrasi 100% sebesar 1 CFU. Konsentrasi efektif perasan daun cengkodok (Melastoma malabathricum L.) dimulai dari konsentrasi 60% dengan hambatan pertumbuhan jamur Trichophyton rubrum sebesar 52.77% dan rebusan dimulai dari konsentrasi 50% dengan persentase hambatan 59.41%. Dianalisis statistik menggunakan uji independent t test didapatkan hasil p value (Sig. 2 tailed) 0.116 > 0.05 yang menunjukkan tidak terdapat perbedaan antara perasan dan rebusan daun cengkodok (Melastoma malabathricum L.) dalam menghambat jamur Trichophyton rubrum.


Keywords


Perasan; Rebusan; Daun Cengkodok; Trichophyton Rubrum

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DOI: https://doi.org/10.30602/jlk.v2i2.336

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