Identifikasi Bakteri Mirip Coliform pada Media Cromocoult Coliform Agar (CCA)

Paulina Rosa Evriarti

Abstract


Coliform-like bacteria can be found when examining coliforms in fresh water using the CCA media on membrane filter method. Therefore, it is necessary to characterize and identify coliform-like colonies that grow on CCA medium so that is easier to perform colony count analysis. One hundred ml of fresh water samples were filtered using filter membrane paper and then planted in CCA medium. Two different colonies growing on the media (red (A) and pink-purple (B)) were subjected to oxidase test, gram staining and re-cultured in various growth media such as blood agar, nutrient agar, MacConkey, and B. coli agar. Both colony types were also identified with Vitek MS to determine the species. The results of the examination showed that the red colonies (code A) were coliform-like bacteria, while the pink-purple colonies (code B) were coliform bacteria. The characteristics of coliform-like bacteria are that they produce hemolysis on blood agar and do not ferment lactose. Identification with Vitek MS showed that the isolated coliform-like bacteria was Aeromonas hydrophilla. Therefore, it can be concluded that Aeromonas sp. are coliform-like bacteria that grow on CCA, so further verification is needed when counting membrane filter colonies using CCA media.

Keywords


CCA, Coliform, Membran Filter Test, Non-Coliform

Full Text:

PDF

References


Aryal, S. (2022a). Blood Agar- Composition, Preparation, Uses and Pictures. Retrieved from https://microbiologyinfo.com/blood-agar-composition-preparation-uses-and-pictures/

Aryal, S. (2022b). Oxidase Test- Principle, Uses, Procedure, Types, Result Interpretation, Examples and Limitations. Retrieved from https://microbiologyinfo.com/oxidase-test-principle-uses-procedure-types-result-interpretation-examples-and-limitations/

Batra, P., Mathur, P., & Misra, M. C. (2016). Aeromonas spp.: an emerging nosocomial pathogen. Journal of laboratory physicians, 8(01), 001-004.

Carranzo, I. V. (2012). Standard Methods for examination of water and wastewater. Paper presented at the Anales De Hidrología Médica.

Carroll, K. C., Butel, J., & Morse, S. (2015). Jawetz melnick and adelbergs medical microbiology 27 E: McGraw-Hill Education.

Chavan, D., Khatoon, H., Anokhe, A., & Kalia, V. (2022). Oxidase test: A biochemical method in bacterial identification.

Fernández-Bravo, A., & Figueras, M. (2020). An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity. Microorganisms, 8, 129. doi:10.3390/microorganisms8010129

Halkman, H. B. D., & Halkman, A. K. (2014). Indicator Organisms. In C. A. Batt & M. L. Tortorello (Eds.), Encyclopedia of Food Microbiology (Second Edition) (pp. 358-363). Oxford: Academic Press.

Lamy, B., & Horneman Amy, J. (2019). Aeromonas.

Lange, B., Strathmann, M., & Oßmer, R. (2013). Performance validation of chromogenic coliform agar for the enumeration of Escherichia coli and coliform bacteria. Letters in applied microbiology, 57(6), 547-553.

MC, E. (2020a). Microbe Canvas: Citrobacter. Retrieved from https://www.microbe-canvas.com/search.php?tags=citrobacter&nc=-1

MC, E. (2020b). Microbe Canvas: Enterobacter

Retrieved from https://www.microbe-canvas.com/search.php?tags=enterobacter&nc=-1

MC, E. (2020c). Microbe Canvas: Klebsiella. Retrieved from https://www.microbe-canvas.com/search.php?tags=klebsiella&nc=-1,

Murray, P. R., Rosenthal, K. S., & Pfaller, M. A. (2020). Medical microbiology E-book: Elsevier Health Sciences.

MuttalibAl-shalah, L. A. (2017). The study characters of Aeromonas hydrophila on some media. Mesopotamia Environmental Journal(Special Issue C).

Ristow, L. C., & Welch, R. A. (2016). Hemolysin of uropathogenic Escherichia coli: A cloak or a dagger? Biochimica et Biophysica Acta (BBA) - Biomembranes, 1858(3), 538-545.

doi:https://doi.org/10.1016/j.bbamem.2015.08.015

Shields, P., & Cathcart, L. (2010). Oxidase test protocol. American Society for Microbiology, 1-9.

SNI. (2015). SNI 3554:2015: Cara uji air minum dalam kemasan. Retrieved from https://vdocuments.site/9402sni-3554-2015.html?page=2

Talaro, K., Chess, B., Wiersema, D. S., & Sen, P. (2013). Foundations in Microbiology, 2012: McGraw-Hill

Tominaga, T., & Ishii, M. (2020). Chapter 11 - Detection of microorganisms with lateral flow test strips. In C. S. Pavia & V. Gurtler (Eds.), Methods in Microbiology (Vol. 47, pp. 351-394): Academic Press.




DOI: https://doi.org/10.30602/jlk.v6i1.1047

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Jurnal Laboratorium Khatulistiwa

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.



JURNAL LABORATORIUM KHATULISTIWA (JLK)

Jurnal Laboratorium Khatulistiwa (JLK) (Print ISSN: 2597-9523 | e-ISSN: 2597-9531 ) is a peer-reviewed open-access scientific journal published by the Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak. This journal focuses on the development of medical laboratory science and technology.



Jurnal Laboratorium Khatulistiwa is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License .
CONTACT
✉ jurnallaboratoriumkhatulistiwa@poltekkes-pontianak.ac.id
📍 Poltekkes Kemenkes Pontianak, Jl. 28 Oktober Siantan Hulu, Pontianak, Kalimantan Barat, Indonesia



COLLABORATION
© Poltekkes Kemenkes Pontianak - Jurnal Laboratorium Khatulistiwa (JLK)