Proteksi Biji Kopi Dalam Memperbaiki Morfologi Sperma Pada Tikus Yang Diinduksi Streptozotocin

Mochamad Rizal Maulana, Eko Naning Sofyanita

Abstract


Pemberian induksi Streptozotocin (Stz) dapat menurunkan kualitas sperma salah satunya morfologi sel spermatozoa, kopi mempunyai kandungan antikosidan yang tinggi yaitu asam klorogenat yang merupakan antioksidan kuat, mampu bertindak sebagai antioksidan eksogen yang melawan ROS dalam sel. Tujuan dari penelitian ini untuk mengatahui pengaruh pemberian kopi pada tikus yang diinduksi Stz. Post Test Control Group Design dengan sampel 24 tikus jantan Rattus norvegicus umur 3 bulan, berat badan ± 250 gram, dibagi menjadi 4 kelompok secara random. Setelah 1 minggu aklimasi, pengobatan diberikan 1 kali sehari selama 14 hari. Hari ke 15 dilakukan pemerkksaan morfologi sperma.. Hasil penelitian morfologi spermatozoa (%) pada kelompok KN (50,46) kelompok KP (64,58) kelompok KK1 (59,50) dan kelompok KK2 (72,05) berbeda artinya, p< 0,05. Kesimpulan pada penelitian ini yaitu Pemberian kopi mampu memperbaiki morfologi spermatozoa pada tikus yang diinduksi Stz.


Keywords


Kopi; Motilitas; Diabetes Melitus

Full Text:

PDF

References


Alves, R. C., Soares, C. M. D., & Oliveira, M. B. P. P. (2015). Isoflavones in Coffee. In Coffee in Health and Disease Prevention. Elsevier Inc. https://doi.org/10.1016/B978-0-12-409517-5.00016-4

Farid, A., Gunawan, I., Asam, I., Dari, K., & Lanang, K. (2022). Tesis Identifikasi Asam Klorogenat Dari “ Kopi Sebagai Sumber Antioksidan untuk mencapai derajat sarjana S-2.

Fitriana, V. N., & Jatmiko, S. W. (2019). Pengaruh Pemberian Kopi Terhadap Morfologi Spermatozoa Tikus Wistar ( Rattus norvegicus ). 462–471.

Ihara, Y., Asahara, S. I., Inoue, H., Seike, M., Ando, M., Kabutoya, H., Kimura-Koyanagi, M., & Kido, Y. (2023). Chlorogenic Acid and Caffeine in Coffee Restore Insulin Signaling in Pancreatic Beta Cells. Kobe Journal of Medical Sciences, 69(1), E1–E8.

Kolb, H., Martin, S., & Kempf, K. (2021). Coffee and lower risk of type 2 diabetes: Arguments for a causal relationship. Nutrients, 13(4), 1–17. https://doi.org/10.3390/nu13041144

La Vignera, S., Condorelli, R., Vicari, E., D’Agata, R., & Calogero, A. E. (2012). Diabetes mellitus and minireview sperm parameters. Journal of Andrology, 33(2), 145–153. https://doi.org/10.2164/jandrol.111.013193

Metro, U. M., & Penelitian, A. H. (2023). 2 1*,2. 5, 64–81.

Ricci, E., Viganò, P., Cipriani, S., Somigliana, E., Chiaffarino, F.,

Bulfoni, A., & Parazzini, F. (2017). Coffee and caffeine intake and male infertility: A systematic review. Nutrition Journal, 16(1), 1–14. https://doi.org/10.1186/s12937-017-0257-2

Sailaxmi, G., & Lalitha, K. (2015). Impact of a stress management program on stress perception of nurses working with psychiatric patients. Asian Journal of Psychiatry, 14(2015), 42–45. https://doi.org/10.1016/j.ajp.2015.01.002

Saputra, N. T., Suartha, I. N., & Dharmayudha, A. A. G. O. (2018). Agen Diabetagonik Streptozotocin untuk Membuat Tikus Putih Jantan Diabetes Mellitus. Buletin Veteriner Udayana, 10(2), 116. https://doi.org/10.24843/bulvet.2018.v10.i02.p02

Scarano, W. R., Messias, A. G., Oliva, S. U., Klinefelter, G. R., & Kempinas, W. G. (2006). Sexual behaviour, sperm quantity and quality after short-term streptozotocin-induced hyperglycaemia in rats. International Journal of Andrology, 29(4), 482–488. https://doi.org/10.1111/j.1365-2605.2006.00682.x

Sofyanita, E. N., & Iswara, A. (2021). Rasio Penutupan Luka pada Tikus Diabetes Diinduksi Streptozotocin dengan Perlakuan Dressing Tipe Pasif dan Interaktif ( Penelitian Pendahuluan ) Wound Closure Ratio in Streptozotocin-Induced Diabetic Mice Treated by Passive and Interactive Dressing ( Pilo. Laboratorium Medis, 03(02), 67–71.

Sofyanita, E. N., Iswara, A., & Riadi, A. (2023). Angiogenesis Stimulation in Bacteria-Infected Acute Wound Healing With Honey Treatment in Balb/C Mice. Journal of Engineering and Technology for Industrial Applications, 9(42), 16–20. https://doi.org/10.5935/jetia.v9i42.869

Sofyanita, E. N., Nasihun, T., & Zulaikhah, S. T. (2019). Eko naning MSG 4033-18343-8-PB. 10(2), 51–56.

Sofyanita, E. N., & Yunianti, A. R. (2023). Effectiveness of White Guava Leaves (Psidium Guajava Var. Pyrifera L.) in Repair Proximal Tubule Damage and Glomerulus Diameter in Hyperglycemic Mice. Journal of Engineering and Technology for Industrial Applications, 9(41), 41–45. https://doi.org/10.5935/jetia.v9i41.863

Wahono, B. (2016). Effects of Peaberry Coffee on The Sexual Behavior and The Blood Testosterone Levels of The Male Mouse ( Mus musculus ). Proceeding Of 3rd International Conference on Research, Implementation and Education of Mathematics and Science Yogyakarta, May, 16–17.

WHO. (2016). Global Report on Diabetes. Isbn, 978(April), 6–86.

Wulandari, S., & Agustin, Y. (2022). Biji Kopi Robusta Peaberry Green Bean : Skrining Fitokimia, Formulasi Herbal Lotion. Journal of Pharmaceutical And Sciences, 5(2), 255–263. https://doi.org/10.36490/journal-jps.com.v5i2.152

Yafi, F. A., Jenkins, L., Albersen, M., Corona, G., Isidori, A. M., Goldfarb, S., Maggi, M., Nelson, C. J., Parish, S., Salonia, A., Tan, R., Mulhall, J. P., & Hellstrom, W. J. G. (2016). Erectile dysfunction. Nature Reviews Disease Primers, 2(1). https://doi.org/10.1038/NRDP.2016.3




DOI: https://doi.org/10.30602/jlk.v7i2.1421

Article Metrics

Abstract views : 203| PDF views : 31

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Jurnal Laboratorium Khatulistiwa

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.