ISOLATION AND IDENTIFICATION OF STAPHYLOCOCCUS AUREUS IN DIABETIC ULCERS AS A RISK FACTOR FOR INFECTION

Winda Irawati Zebua; Maniur Arianto Siahaan; Apriska Dewi Sipayung; Ade Indah N Sipahutar

doi:10.30602/jvk.v12i1.2111

ISOLATION AND IDENTIFICATION OF STAPHYLOCOCCUS AUREUS IN DIABETIC ULCERS AS A RISK FACTOR FOR INFECTION

Winda Irawati Zebua, Maniur Arianto Siahaan, Apriska Dewi Sipayung, Ade Indah N Sipahutar

Abstract

Diabetic foot ulcers are chronic complications of diabetes mellitus prone to bacterial infection, increasing the risk of amputation and mortality. This study aims to analyze the relationship between patient characteristics and the presence of Staphylococcus aureus in diabetic ulcers as a risk factor for infection. This study used an analytic cross-sectional design and was conducted at Bunda Thamrin Clinic Laboratory, Medan, in April 2025. A total of 30 diabetic foot ulcer samples were collected using total sampling. Bacterial cultures were grown on Blood Agar, followed by Gram staining, catalase, coagulase tests, and identification using the Vitek 2 Compact system. Statistical analysis was performed using Chi-square and Spearman correlation tests with a significance level of p<0.05. Of the 30 samples examined, Staphylococcus aureus was found in 14 (46.7%) cases, Staphylococcus epidermidis in 6 (20%), Citrobacter freundii in 5 (16.7%), and Proteus mirabilis in 5 (16.7%). The Chi-square test showed a significant association between gender and S. aureus infection (p=0.042) and between age group and infection (p=0.031). The Spearman test showed a positive correlation between fasting blood glucose level and the presence of S. aureus (r=0.428; p=0.018). There is a significant relationship between patient characteristics (gender, age, and blood glucose level) and staphylococcus aureus infection in diabetic ulcers. Early bacterial identification is crucial for targeted antibiotic therapy and prevention of complications such as osteomyelitis and amputation.

Keywords

Ulcers; Diabetes; Staphylococcus aureus; Infection.

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DOI: https://doi.org/10.30602/jvk.v12i1.2111