Analisis Gen ORF dan Gen N Pada Pasien Terkonfirmasi Covid19 Kategori Positif Tinggi di Kalimantan Barat
Abstract
Covid19 merupakan pandemic dunia yang disebabkan oleh virus Sar-Cov-2. Virus ini mulai masuk dan menyebar di Kalimantan Barat sejak April 2020. Pada penelitian ini dilakukan analisis deteksi virus Sar-Cov-2 pada pasien dengan kategori positif tinggi dengan target gen ORF dan gen N. Hasil Swab Naso/Oro pasien dianalisis dengan menggunakan Real Time Polymerase Chain Reaction (RT-PCR). Hasil penelitian menujukkan bahawa analisis dengan kedua gen menghasilkan nilai Ct dan Viral Load yang bervariasi. Ct rendah terkait dengan Viral Load yang tinggi namun hal ini tidak berkorelasi absolut. Selain itu analisis sampel dari bulan Mei hingga November 2020 juga menunjukkan adanya peningkatan viral load sampel yang mungkin terjadi akibat mutase gen D614G.
Keywords
Full Text:
PDFReferences
Archived: WHO Timeline - COVID-19. (n.d.). Retrieved November 13, 2020, from https://www.who.int/news/item/27-04-2020-who-timeline---covid-19
Chang, M. C., Hur, J., & Park, D. (2020). Interpreting the COVID-19 Test Results: A Guide for Physiatrists. American Journal of Physical Medicine & Rehabilitation. https://doi.org/10.1097/PHM.0000000000001471
Coronavirus Update (Live): 53,109,704 Cases and 1,299,651 Deaths from COVID-19 Virus Pandemic - Worldometer. (n.d.). Retrieved November 13, 2020, from https://www.worldometers.info/coronavirus/
Fu, L., Wang, B., Yuan, T., Chen, X., Ao, Y., Fitzpatrick, T., Li, P., Zhou, Y., Lin, Y., Duan, Q., Luo, G., Fan, S., Lu, Y., Feng, A., Zhan, Y., Liang, B., Cai, W., Zhang, L., Du, X., … Zou, H. (2020). Clinical characteristics of coronavirus disease 2019 (COVID-19) in China: A systematic review and meta-analysis. The Journal of Infection, 80(6), 656–665. https://doi.org/10.1016/j.jinf.2020.03.041
Genomic characterisation and epidemiology of 2019 novel coronavirus: Implications for virus origins and receptor binding—The Lancet. (n.d.). Retrieved November 13, 2020, from https://www.thelancet.com/article/S0140-6736(20)30251-8/fulltext
GISAID - Initiative. (n.d.). Retrieved November 13, 2020, from https://www.gisaid.org/
Hamming, I., Timens, W., Bulthuis, M., Lely, A., Navis, G., & van Goor, H. (2004). Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. The Journal of Pathology, 203(2), 631–637. https://doi.org/10.1002/path.1570
Hu, W., Bai, B., Hu, Z., Chen, Z., An, X., Tang, L., Yang, J., Wang, H., & Wang, H. (2005). Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets. Journal of Clinical Microbiology, 43(5), 2041–2046. https://doi.org/10.1128/JCM.43.5.2041-2046.2005
Kakhki, R. K., Kakhki, M. K., & Neshani, A. (2020). COVID-19 target: A specific target for novel coronavirus detection. Gene Reports, 20, 100740. https://doi.org/10.1016/j.genrep.2020.100740
Kawase, J., Asakura, H., Kurosaki, M., Oshiro, H., Etoh, Y., Ikeda, T., Watahiki, M., Kameyama, M., Hayashi, F., Kawakami, Y., Murakami, Y., & Tsunomori, Y. (2018). Rapid and Accurate Diagnosis Based on Real-Time PCR Cycle Threshold Value for the Identification of Campylobacter jejuni, astA Gene-Positive Escherichia coli, and eae Gene-Positive E. coli. Japanese Journal of Infectious Diseases, 71(1), 79–84. https://doi.org/10.7883/yoken.JJID.2017.151
Korber, B., Fischer, W. M., Gnanakaran, S., Yoon, H., Theiler, J., Abfalterer, W., Hengartner, N., Giorgi, E. E., Bhattacharya, T., Foley, B., Hastie, K. M., Parker, M. D., Partridge, D. G., Evans, C. M., Freeman, T. M., de Silva, T. I., Sheffield COVID-19 Genomics Group, McDanal, C., Perez, L. G., … Montefiori, D. C. (2020). Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus. Cell, 182(4), 812-827.e19. https://doi.org/10.1016/j.cell.2020.06.043
Malik, Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology, 42(1), 3–11.
OASH-nasal-specimen-collection-fact-sheet.pdf. (n.d.). Retrieved November 13, 2020, from https://www.cdc.gov/coronavirus/2019-ncov/downloads/OASH-nasal-specimen-collection-fact-sheet.pdf
QIAamp Viral RNA Mini Handbook—QIAGEN. (n.d.). Retrieved November 13, 2020, from https://www.qiagen.com/us/resources/resourcedetail?id=c80685c0-4103-49ea-aa72-8989420e3018〈=en
Real-Time PCR & qRT-PCR – SolGent Co., Ltd. (n.d.). Retrieved November 13, 2020, from http://solgent.com/english/sub03010103
Suryadi, A. D., Ridho, I., & Suryadarma. (2020). The Impact of COVID-19 Outbreak on Poverty: An Estimation for Indonesia. SMERU. http://smeru.or.id/sites/default/files/publication/wp_covid19impact_draft.pdf.
The Genome Sequence of the SARS-Associated Coronavirus | Science. (n.d.). Retrieved November 14, 2020, from https://science.sciencemag.org/content/300/5624/1399.abstract
User Guide: Maxima H Minus ds cDNA Synthesis Kit. (n.d.). Retrieved November 13, 2020, from https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2FMAN0012724_Maxima_H_Minus_ds_cDNA_Syn_UG.pdf&title=VXNlciBHdWlkZTogTWF4aW1hIEggTWludXMgZHMgY0ROQSBTeW50aGVzaXMgS2l0
Whole-genome sequencing reveals genomic signatures associated with the inflammatory microenvironments in Chinese NSCLC patients | Nature Communications. (n.d.). Retrieved November 13, 2020, from https://www.nature.com/articles/s41467-018-04492-2
DOI: https://doi.org/10.30602/jvk.v7i1.629
Article Metrics
Abstract views : 507|
PDF views : 3
Refbacks
- There are currently no refbacks.
Copyright (c) 2022 Jurnal Vokasi Kesehatan
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
JVK (JURNAL VOKASI KESEHATAN) INDEXED BY:
Â
 
  
 
 
 
 
  
LICENSE JOURNAL:
Jurnal Vokasi Kesehatan (JVK) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
MAPS: